Monoclonal Antibodies to Detect SARS-CoV1 & SARS-CoV2

Technology Overview

COVID-19 is caused by infection with SARS-CoV2.

Two mouse monoclonal antibodies (mAb’s) are presented, one which specifically binds to the Spike protein (S) and the other which specifically binds to the Nucleocapsid protein (NP), both of which recognize SARS-CoV1 & SARS-CoV2, but do not bind to other beta-coronaviruses.

Stage of Development

• Two mouse monoclonal antibodies that are specific to the Spike protein and Nucleocapsid protein as measured in Western Blot, ELISA and flow cytometry
• One mouse monoclonal antibody that binds specifically to spike of SARs-CoV1 and neutralizes SARS-CoV1

Benefits

• The mAb’s can serve as controls for testing of serum from patients for the presence of antibodies to SARs-CoV2
• Both the Spike and NP can be used to stain for the presence of virus in potentially infected cells
• For serum neutralization the NP antibody is best used to determine if virus has infected cells or been blocked by patients’ serum antibodies

Applications

• Detecting virus in saliva or other human secretions such as stool
• Immunohistochemical staining of tissues for presence of the virus
• Western blots looking for presence of virus proteins from infected cell lysates
• Capture antibody in bead-based ELISA’s
• Controls for all types of SARs-CoV2 (or SARs-CoV1) assays