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RNA-Targeting CAS9 System for Controlling Viruses

SauCas9 offers the advantage of repressing viruses whose lifecycles consist of solely RNA molecules, thereby inaccessible to DNA cleavage

Background

CRISPR Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9) is a protein that plays an important role in immunological defense of bacteria against DNA viruses. A CRISPR-Cas based system can be used to target nucleic acid sequences. Cas9 is used frequently in genetic engineering. The protein functions by cutting DNA to alter a cell’s genome. Cas9 can be used to perform site-directed double strand breaks in DNA. The breaks that occur can cause inactivation of some genes or introduction of others. Cas9 is typically guided by RNA and it identifies sections of DNA that are to be removed and removes them. Cas9 has focused on its function as a DNA endonuclease, however the cleaving of RNA has

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