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Mouse Monoclonal Antibodies Targeting SARS-CoV-2 Nucleocapsid Protein

Monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid protein for use in diagnostic assays

Background

Coronavirus nucleocapsid protein plays a key role in coating the viral RNA genome during virion formation, and overall pathogenesis. Additionally, the nucleocapsid protein has been used as a diagnostic marker for active coronavirus infection. There are very few antibody reagents currently available that are specific for SARS-CoV-2 nucleocapsid, as most monoclonal antibodies that are commercially available were generated against SARS-CoV nucleocapsid protein and are cross-reactive for SARS-CoV-2 nucleocapsid.

Technology Overview

Researchers at Colorado State University have developed several mouse monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid protein.

Highlights:

  • Clones were screened by ELISA for reactivity towards recombinant SARS-CoV-2 nucleocapsid protein, counter screened against bacterial lysates and heterologous 6xHis-tagged proteins
  • Top 18 clones were tested for efficacy in Western blot

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