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Virus Detection through Particle Counting on Paper Microfluidics using a Smartphone-Based Fluorescence Microscope

A smartphone-based fluorescence microscope and an image-processing algorithm are used to detect norovirus, and potentially COVID-19

Background

Noroviruses are a group of non-enveloped, single-stranded RNA viruses that can cause inflammation of the stomach or intestines. They are human enteric viruses that can be highly infectious upon ingestion of low doses of virions. In principle, norovirus can be detected in water, food, and environmental specimens. However, this current process involves complicated concentration of viruses and/or amplification of the norovirus genome, thus the detection is almost impossible in field settings.

Technology Overview

The inventors have performed norovirus detection on a microfluidic paper analytic device without using any sample concentration or nucleic acid amplification steps. The technology uses a smartphone-based fluorescence microscope and an image-processing algorithm to isolate the particles aggregated by antibody-antigen binding. This leads to a limit

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