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Efficient Identification of Active Ingredients Against SARS-CoV-2 and Chemical Probes as Inhibitors of the 3CL and PL Proteases of Coronaviruses

Simple and effective screening method for the identification of cell-compatible drugs against viruses such as SARS-CoV-2

Background

The search for an effective COVID-19 drug is in global focus facing the prevailing pandemic. Several open reading frames have already been identified in the genome of SARS-CoV-2, which code for proteins that are crucial for the replication of the virus. In order to use these proteins, the polyprotein must be cleaved into the individual functional proteins by the proteases PLpro and 3CLpro. These proteases are therefore a primary target for drug development.

Up to now, classical enzyme assays with fluorogenic substrate have been used for the identification of drug candidates. However, this multi-step procedure can only be used for in vitro studies – potential candidates must be examined in a subsequent step with regard to important aspects such

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