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Plasmid to Produce Recombinant SARS-CoV-2 Nucleocapsid Protein

A plasmid and purification protocol for the production of a soluble nucleocapsid protein, essential for developing COVID-19 diagnostics

Technology Overview

Researchers at Colorado State University have developed a plasmid and purification protocol for the expression and purification of a soluble SARS-CoV-2 nucleocapsid protein for use in diagnostic assays, in response to the SARS-CoV-2/COVID-19 outbreak.

Details:

  • Bacterial expression plasmid encodes a bacterial codon-optimized fragment of the SARS-CoV-2 nucleocapsid protein
  • Plasmid was sequence verified
  • Bacterial expression protocol can produce monomeric nucleocapsid protein (as confirmed by mass spectroscopy) with greater than 95% purify
  • Purified nucleocapsid protein has been tested by collaborators for antigenicity in mice immunized against the recombinant protein as well as bats infected with SARS-CoV-2
  • Protein expressed and purified from this plasmid is antigenic
  • Protein can be used for ELISAs as well as other assays to indicate presence

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